Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.

@article{Srensen1993AmplificationAS,
  title={Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.},
  author={Annette Balle S\orensen and Mogens Ryttergaard Duch and Poul J\orgensen and Finn Skou Pedersen},
  journal={Journal of virology},
  year={1993},
  volume={67 12},
  pages={7118-24}
}
We describe a two-step polymerase chain reaction method that can be used for the amplification of cellular DNA sequences adjacent to an integrated retroviral provirus. The technique involves a partly degenerate, arbitrary primer that will hybridize in the provirus-flanking cellular DNA. By using this primer in combination with a biotinylated provirus-specific primer, a provirus-cellular DNA junction fragment can be isolated from the nonspecific amplification products by using streptavidin… CONTINUE READING

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