Amplification and cloning of complete enterovirus genomes by long distance PCR.

@article{Lindberg1997AmplificationAC,
  title={Amplification and cloning of complete enterovirus genomes by long distance PCR.},
  author={A Michael Lindberg and Charlotta Polacek and Susanne Johansson},
  journal={Journal of virological methods},
  year={1997},
  volume={65 2},
  pages={191-9}
}
A method for amplification and cloning of complete enterovirus cDNA genomes is described. Viral RNA was reverse transcribed using an optimized protocol and a reverse transcriptase with reduced RNase H activity. Amplicons corresponding to complete genomes of 14 prototype strains of group B coxsackieviruses and echoviruses were amplified using oligonucleotide primers derived from the Coxsackievirus B3 genomic sequence of the 5' and 3' ends and a mixture of thermostable DNA polymerases… CONTINUE READING

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