Amplifiable DNA from gram-negative and gram-positive bacteria by a low strength pulsed electric field method.

Abstract

An efficient electric field-based procedure for cell disruption and DNA isolation is described. Isoosmotic suspensions of Gram-negative and Gram-positive bacteria were treated with pulsed electric fields of <60 V/cm. Pulses had an exponential decay waveform with a time constant of 3.4 micros. DNA yield was linearly dependent on time or pulse number, with… (More)

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Cite this paper

@article{Vitzthum2000AmplifiableDF, title={Amplifiable DNA from gram-negative and gram-positive bacteria by a low strength pulsed electric field method.}, author={Frank Vitzthum and Gebhard Geiger and Hans Bisswanger and B Elkine and Hermann Brunner and J{\"{u}rrgen Bernhagen}, journal={Nucleic acids research}, year={2000}, volume={28 8}, pages={E37} }