Amphipols Outperform Dodecylmaltoside Micelles in Stabilizing Membrane Protein Structure in the Gas Phase

@inproceedings{Calabrese2015AmphipolsOD,
  title={Amphipols Outperform Dodecylmaltoside Micelles in
Stabilizing Membrane Protein Structure in the Gas Phase},
  author={Antonio N. Calabrese and Thomas G. Watkinson and Peter J F Henderson and Sheena E Radford and Alison. E. Ashcroft},
  booktitle={Analytical chemistry},
  year={2015}
}
Noncovalent mass spectrometry (MS) is emerging as an invaluable technique to probe the structure, interactions, and dynamics of membrane proteins (MPs). However, maintaining native-like MP conformations in the gas phase using detergent solubilized proteins is often challenging and may limit structural analysis. Amphipols, such as the well characterized A8-35, are alternative reagents able to maintain the solubility of MPs in detergent-free solution. In this work, the ability of A8-35 to retain… CONTINUE READING
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