Amphipathic homopolymers for siRNA delivery: probing impact of bifunctional polymer composition on transfection.

@article{Buerkli2014AmphipathicHF,
  title={Amphipathic homopolymers for siRNA delivery: probing impact of bifunctional polymer composition on transfection.},
  author={Christian Buerkli and Soo Hyeon Lee and Elena V. Moroz and Mihaiela C. Stuparu and Jean‐Christophe Leroux and Anzar Khan},
  journal={Biomacromolecules},
  year={2014},
  volume={15 5},
  pages={
          1707-15
        }
}
In this study, we systematically explore the influence of the lipophilic group on the siRNA transfection properties of the polycationic-based delivery vectors. For this, a novel and modular synthetic strategy was developed for the preparation of polymers carrying a cationic site and a lipophilic group at each polymer repeat unit. These bifunctional polymers could form a complex with siRNA and deliver it to human colon carcinoma cells (HT-29-luc). In general, transfection capability increased… 

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References

SHOWING 1-10 OF 69 REFERENCES

Glycine-terminated dendritic amphiphiles for nonviral gene delivery.

This study has developed multivalent polyglycerol dendron based amphiphiles with well-defined molecular structures that express controlled glycine arrays on their surfaces that acts as an efficient vector to deliver siRNA and achieve potent gene silencing by investigating the knockdown of luciferase and GAPDH gene activity in HeLa cells.

Development of a novel endosomolytic diblock copolymer for siRNA delivery.

The effect and role of carbon atoms in poly(β-amino ester)s for DNA binding and gene delivery.

It is observed in both cell lines that the PBAE-DNA binding constant is biphasic with the transfection efficacy and that the optimal values of the binding constant with respect to the transfecture efficacy are in the range (1-6) × 10(4) M(-1).

Hydrophobically modified oligoethylenimines as highly efficient transfection agents for siRNA delivery.

A remarkable increase of biocompatibility without loss of efficiency could be achieved by coformulation of OEI-HA-10 with lauryl acrylate modified OEi-LA-5.

Comparative study of guanidine-based and lysine-based brush copolymers for plasmid delivery.

The goal of this work is to investigate the effect of guanidinylation on gene transfer ability of HPMA-co-oligolysine copolymers and find the best performingCopolymers provided more efficient gene transfection with less associated cytotoxicity than PEI.

Polyphosphonium polymers for siRNA delivery: an efficient and nontoxic alternative to polyammonium carriers.

The present results show that the use of positively charged phosphonium groups is a worthy choice to achieve a good balance between toxicity and transfection efficiency in gene delivery systems.

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.

Alkane-modified short polyethyleneimine for siRNA delivery.

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