Amphipathic helical behavior of the third repeat fragment in the tau microtubule-binding domain, studied by (1)H NMR spectroscopy.

@article{Minoura2002AmphipathicHB,
  title={Amphipathic helical behavior of the third repeat fragment in the tau microtubule-binding domain, studied by (1)H NMR spectroscopy.},
  author={Katsuhiko Minoura and Koji Tomoo and Toshimasa Ishida and Hiroshi Hasegawa and Masahiro Sasaki and Taizo Taniguchi},
  journal={Biochemical and biophysical research communications},
  year={2002},
  volume={294 2},
  pages={210-4}
}
The third repeat fragment (3MBD, 31 residues) in the four-repeat microtubule-binding domain of water-soluble tau protein has been considered to be responsible for the formation of the neuropathological filament. To clarify the structural requisite of 3MBD for the filamentous assembly, the solution structures in water and trifluoroethanol (TFE) were investigated by a combination of two-dimensional (1)H-NMR measurements and molecular modeling calculations. All protons were assigned by various 2D… CONTINUE READING