Amperometric sensing at high temperature with a "wired" thermostable glucose-6-phosphate dehydrogenase from Aquifex aeolicus.

@article{Iyer2003AmperometricSA,
  title={Amperometric sensing at high temperature with a "wired" thermostable glucose-6-phosphate dehydrogenase from Aquifex aeolicus.},
  author={Ramesh B. Iyer and Valeri Pavlov and Ioanis Katakis and Leonidas G Bachas},
  journal={Analytical chemistry},
  year={2003},
  volume={75 15},
  pages={3898-901}
}
An amperometric enzyme sensor capable of operating at high temperatures was developed by utilizing a "wired" thermostable glucose-6-phosphate dehydrogenase (tG6PDH) from the hyperthermophilic bacterium Aquifex aeolicus. The response of the system was monitored through detection of the product of the enzymatic reaction, NADH, which was electrocatalytically reoxidized to NAD by a thermostable redox mediator, osmium (1,10-phenanthroline-5,6-dione)2-poly(4-vinylpyridine), at Eapp = +150 mVvs Ag… CONTINUE READING