Amperometric immunoassay for the obesity biomarker amylin using a screen printed carbon electrode functionalized with an electropolymerized carboxylated polypyrrole

@article{MartnezGarca2018AmperometricIF,
  title={Amperometric immunoassay for the obesity biomarker amylin using a screen printed carbon electrode functionalized with an electropolymerized carboxylated polypyrrole},
  author={Gonzalo Mart{\'i}nez-Garc{\'i}a and Esther S{\'a}nchez-Tirado and Araceli Gonz{\'a}lez-Cort{\'e}s and Paloma Y{\'a}{\~n}ez‐Sede{\~n}o and Jos{\'e} Manuel Pingarr{\'o}n},
  journal={Microchimica Acta},
  year={2018},
  volume={185},
  pages={1-8}
}
AbstractAmylin (the islet amyloid polypeptide) is a hormone related to adiposity, hunger and satiety. It is co-secreted with insulin from pancreatic B-cells. An amperometric immunosensor is presented here for the determination of amylin. It is making use of a screen printed carbon electrode (SPCE) functionalized with electropolymerized poly(pyrrole propionic acid) (pPPA) with abundant carboxyl groups that facilitate covalent binding of antibody against amylin. A competitive immunoassay was… 
Current Advances in Electrochemical Biosensors and Nanobiosensors
TLDR
In this review, the recent studies of electrochemical biosensors have been presented with their applications at various fields and nanomaterials and polymers have been used to increase the performance of biosensor.
Metal Nanoparticle and Quantum Dot Tags for Signal Amplification in Electrochemical Immunosensors for Biomarker Detection
With the increasing importance of healthcare and clinical diagnosis, as well as the growing demand for highly sensitive analytical instruments, immunosensors have received considerable attention. In
A liquid crystal based method for detection of urease activity and heavy metal ions by using stimulus-responsive surfactant-encapsulated phosphotungstate clusters
TLDR
The method is highly sensitive and can detect urease activities as low as 0.03 mU/mL and can also be applied to the determination of heavy metal ions which exert an inhibitory effect on the activity of Urease.
A Review: New Trends in Electrode Systems for Sensitive Drug and Biomolecule Analysis
TLDR
In this review, the recent electrochemical drug and biomolecule (DNA, RNA, µRNA, Biomarkers, etc.) studies will be presented that involve new trend disposable electrodes.
A Nuclease Protection ELISA Assay for Colorimetric and Electrochemical Detection of Nucleic Acids.
TLDR
This work describes a nuclease protection enzyme-linked immunosorbent assay (NP-ELISA) for accurate and sensitive detection of nucleic acid and serves as a stepping stone towards developing miniaturized, multiplexed nucleasing protection assays for point-of-care diagnosis.
A Simplified and Robust Activation Procedure of Glass Surfaces for Printing Proteins and Subcellular Micropatterning Experiments
TLDR
A simplified activation procedure for untreated solid substrates based on a commercial polymer metal ion coating (AnteoBindTM Biosensor reagent) that allows for direct µCP and the strong attachment of proteins via avidity binding is presented.
Use of Cysteamine and Glutaraldehyde Chemicals for Robust Functionalization of Substrates with Protein Biomarkers—An Overview on the Construction of Biosensors with Different Transductions
TLDR
The present overview summarizes the pre-functionalization of standard and nanostructured solid/flexible supports with cysteamine (Cys) and glutaraldehyde (GA) chemicals for robust protein immobilization and detection of biomarkers in body fluids using three transductions: piezoelectrical, electrochemical, and optical, respectively.

References

SHOWING 1-10 OF 19 REFERENCES
Development of sensitive immunoassays to detect amylin and amylin-like peptides in unextracted plasma.
TLDR
Two sandwich-type immunoassays that use pairs of monoclonal antibodies, the fluorescent substrate 4-methylumbelliferyl phosphate, and the enzyme alkaline phosphatase are reported here that measures only human amylin; the other also detects amylIn-like peptides.
Colorimetric determination of islet amyloid polypeptide fibrils and their inhibitors using resveratrol functionalized gold nanoparticles
TLDR
A colorimetric method based on the specific recognition of resveratrol by IAPP fibrils that is simple and effective, and therefore is perceived to represent a promising scheme for determination of fibrillar IAPPs and their inhibitors.
Effects of Meal Ingestion on Plasma Amylin Concentration in NIDDM and Nondiabetic Humans
TLDR
It is concluded that amylin is secreted in response to ingestion of either glucose or a mixed meal and circulates at concentrations that do not differ in patients with NIDDM and nondiabetic subjects.
Amylin/islet amyloid polypeptide: biochemistry, physiology, patho-physiology.
TLDR
Amylin is a 37 amino-acid peptide mainly produced by the islet beta-cell and, in muscle, amylin increases lactate output by muscle and increases the plasma lactate concentration, which may serve as a gluconeogenic substrate for the liver, contributing to replenish depleted glycogen stores and to increase glucose production.
The aggregation potential of human amylin determines its cytotoxicity towards islet β‐cells
TLDR
Electro microscopy, thioflavin T fluorescence and CD spectroscopy are employed to analyze amylin preparations whose cytotoxic potential was established by live–dead assay in cultured β‐cells, finding that mature amylIn fibrils are not toxic to β‐ cells, and aggregates of fibril such as occur in pancreatic islet amyloid in vivo are unlikely to contribute toβ‐cell loss.
Basal and stimulated plasma levels of pancreatic amylin indicate its co-secretion with insulin in humans
TLDR
It is concluded, that amylin and insulin are co-secreted in humans, and that the amylIn release is under feedback-control by insulin.
...
...