Amniotic membrane as a substrate for cultivating limbal corneal epithelial cells for autologous transplantation in rabbits.

@article{Koizumi2000AmnioticMA,
  title={Amniotic membrane as a substrate for cultivating limbal corneal epithelial cells for autologous transplantation in rabbits.},
  author={Noriko Koizumi and Tsutomu Inatomi and Andrew J Quantock and Nigel J Fullwood and Atsuyoshi Dota and Shigeru Kinoshita},
  journal={Cornea},
  year={2000},
  volume={19 1},
  pages={65-71}
}
PURPOSE To examine the viability of using human amniotic membrane as substrate for culturing corneal epithelial cells and transplanting them onto severely injured rabbit eyes. METHODS An ocular-surface injury was created in the right eye of eight rabbits by a lamellar keratectomy extending 5 mm outside the limbus. Next, from the limbal region of the uninjured left eyes of five of these animals, a small biopsy of corneal epithelial cells was taken and cultured on acellular human amniotic… CONTINUE READING

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