Ammonium chloride exposure inhibits cytokine-mediated eosinophil survival.

  title={Ammonium chloride exposure inhibits cytokine-mediated eosinophil survival.},
  author={Misao Ide and Deborah A. Weiler and Hirohita Kita and Gerald J. Gleich},
  journal={Journal of immunological methods},
  volume={168 2},
To study human eosinophils, their efficient purification from peripheral blood is crucial. Although a number of purification procedures, including discontinuous Percoll and metrizamide density gradient centrifugation, have been used, it has been difficult to isolate eosinophils from normal donors with consistently high yields and purities. Recently, a new isolation technique called magnetic cell separation system (MACS) was reported. To evaluate this procedure, we isolated eosinophils from… CONTINUE READING


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