Aminoglycoside resistance resulting from tight drug binding to an altered aminoglycoside acetyltransferase.

@article{Magnet2003AminoglycosideRR,
  title={Aminoglycoside resistance resulting from tight drug binding to an altered aminoglycoside acetyltransferase.},
  author={Sophie J Magnet and T Smith and Renjian Zheng and Patrice L. Nordmann and John S Blanchard},
  journal={Antimicrobial agents and chemotherapy},
  year={2003},
  volume={47 5},
  pages={1577-83}
}
The aacA29b gene, which confers an atypical aminoglycoside resistance pattern to Escherichia coli, was identified on a class 1 integron from a multidrug-resistant isolate of Pseudomonas aeruginosa. On the basis of amino acid sequence homology, it was proposed that the gene encoded a 6'-N-acetyltransferase. The resistance gene was cloned into the pET23a(+) vector, and overexpression conferred high-level resistance to the usual substrates of the aminoglycoside N-acetyltransferase AAC(6')-I… CONTINUE READING
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