Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols

@article{Padilla2010AminoglycosideAS,
  title={Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols},
  author={Isabel Maria Gonzalez Padilla and Lorenzo Burgos},
  journal={Plant Cell Reports},
  year={2010},
  volume={29},
  pages={1203-1213}
}
Plant transformation protocols generally involve the use of selectable marker genes for the screening of transgenic material. The bacterial gene nptII, coding for a neomycin phosphotransferase, and the hpt gene, coding for a hygromycin phosphotransferase, are frequently used. These enzymes detoxify aminoglycoside antibiotics by phosphorylation, thereby permitting cell growth in the presence of antibiotics. Nevertheless, the screening for transgenic regenerated shoots is often partial and… CONTINUE READING

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