• DOI:10.1007/s00203-014-1066-z
• Corpus ID: 253722524

Aminocellalysinolytica gen. nov., sp. nov., a l-lysine-degrading, strictly anaerobic bacterium in the class Clostridia isolated from a methanogenic reactor of cattle farms

@article{Ueki2014AminocellalysinolyticaGN,
  title={Aminocellalysinolytica gen. nov., sp. nov., a l-lysine-degrading, strictly anaerobic bacterium in the class Clostridia isolated from a methanogenic reactor of cattle farms},
  author={Atsuko Ueki and Toru Shibuya and Nobuo Kaku and Katsuji Ueki},
  journal={Archives of Microbiology},
  year={2014},
  volume={197},
  pages={97 - 104}
}

• A. Ueki, T. Shibuya, +1 author K. Ueki
• Published 2 December 2014
• Biology
• Archives of Microbiology

A strictly anaerobic bacterial strain (WN037T) was isolated from a methanogenic reactor. Cells were Gram-positive rods. Strain WN037T was asaccharolytic. The strain fermented l-lysine in the presence of B-vitamin mixture or vitamin B12 and produced acetate and butyrate. l-arginine and casamino acids poorly supported the growth. Strain WN037T used neither other amino acids nor organic acids examined. The strain had C18:1 ω7c, C16:0 and C18:1 ω7c DMA as the predominant cellular fatty acids. The genomic DNA G + C content was 44.2 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain WN037T in the family Eubacteriaceae in the class Clostridia. The closest relative was Eubacterium pyruvativorans (sequence similarity, 92.8 %). Based on the comprehensive analyses, the novel genus and species, Aminocellalysinolytica gen. nov., sp. nov. was proposed to accommodate the strain. The type strain is WN037T (= JCM 19863T = DSM 28287T).