Aminoalkylindole binding in rat cerebellum: selective displacement by natural and synthetic cannabinoids.

@article{Kuster1993AminoalkylindoleBI,
  title={Aminoalkylindole binding in rat cerebellum: selective displacement by natural and synthetic cannabinoids.},
  author={Joan E. Kuster and Jim Stevenson and Stephen John Ward and T E D'Ambra and D. A. Haycock},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={1993},
  volume={264 3},
  pages={1352-63}
}
A binding assay for WIN 55212-2, an aminoalkylindole (AAI) with antinociceptive activity in rodents, is described. [3H]WIN 55212-2 bound to rat cerebellar membranes with a Kd of 2 nM and a maximum binding of 1.2 pmol/mg of protein. Specific binding in this filtration assay was greater than 90%, saturable, reversible, stereospecific, pH sensitive and heat… CONTINUE READING