Amino terminal glycation of gastric inhibitory polypeptide enhances its insulinotropic action on clonal pancreatic B-cells.

@article{OHarte1998AminoTG,
  title={Amino terminal glycation of gastric inhibitory polypeptide enhances its insulinotropic action on clonal pancreatic B-cells.},
  author={Finbarr P M O'Harte and Yasser Hassan Atef Abdel-Wahab and J Michael Conlon and Peter Raymond Flatt},
  journal={Biochimica et biophysica acta},
  year={1998},
  volume={1425 2},
  pages={319-27}
}
Gastric inhibitory polypeptide (GIP) is a potent insulin-releasing hormone of the enteroinsular axis. This study has examined glycation of GIP and effects of such structural modification on insulin secretion from a glucose-responsive clonal pancreatic B-cell line (BRIN-BD11). Monoglycated GIP (Mr 5149.5) was prepared by incubation with d-glucose under reducing conditions and purified by HPLC. Automated Edman degradation and mass spectrometric analysis indicated that GIP was specifically… CONTINUE READING