Amino-bonded silica as stationary phase for liquid chromatographic determination of cyclopiazonic acid in fungal extracts.

@article{Monaci2002AminobondedSA,
  title={Amino-bonded silica as stationary phase for liquid chromatographic determination of cyclopiazonic acid in fungal extracts.},
  author={Linda Monaci and Antonella Aresta and Francesco Palmisano and Angelo Visconti and Carlo Giorgio Zambonin},
  journal={Journal of chromatography. A},
  year={2002},
  volume={955 1},
  pages={79-86}
}
A new high-performance liquid (HPLC) chromatographic method is described for cyclopiazonic acid (CPA) determination in fungal cultures on a propylamino-bonded stationary phase with a CH3CN/CH3COONH4 buffer as mobile phase. Retention of CPA on propylamino modified silica under acidic conditions (protonated amino groups and deprotonated CPA) is governed by a mixed ion-exchange-reversed-phase mechanism. In addition to non-polar (hydrophobic) interactions, polar interactions with the surface… CONTINUE READING