Amino acids interference on the quantification of reducing sugars by the 3,5-dinitrosalicylic acid assay mislead carbohydrase activity measurements.

@article{Teixeira2012AminoAI,
  title={Amino acids interference on the quantification of reducing sugars by the 3,5-dinitrosalicylic acid assay mislead carbohydrase activity measurements.},
  author={Ricardo Sposina Sobral Teixeira and Ayla Sant'Ana da Silva and Viridiana Santana Ferreira-Leit{\~a}o and Elba Pinto da Silva Bon},
  journal={Carbohydrate research},
  year={2012},
  volume={363},
  pages={33-7}
}
This study evaluated the interference of the amino acids tryptophan, cysteine, histidine, tyrosine, hydroxyproline, leucine, proline, serine, glycine, valine, glutamic acid, phenylalanine, and methionine on the measurement of reducing sugars using a phenol-free 3,5-dinitrosalicylic acid (DNS) reagent. It was found that in reaction mixtures containing 20mM of either tryptophan, cysteine, histidine, tyrosine, or hydroxyproline the measurement of 3.7 mM glucose was overestimated by 76%, 50%, 35… CONTINUE READING
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