Amino acid substitutions that specifically impair the transcriptional activity of papillomavirus E2 affect binding to the long isoform of Brd4.

@article{Snchal2007AminoAS,
  title={Amino acid substitutions that specifically impair the transcriptional activity of papillomavirus E2 affect binding to the long isoform of Brd4.},
  author={H{\'e}l{\`e}ne S{\'e}n{\'e}chal and Guy G. Poirier and Benoit Coulombe and Laimonis Laimins and Jacques Archambault},
  journal={Virology},
  year={2007},
  volume={358 1},
  pages={10-7}
}
The E2 protein of papillomaviruses binds to specific sites in the viral genome to regulate its transcription, replication and segregation in mitosis. Amino acid substitutions in the transactivation domain (TAD) of E2, of Arg37 and Ile73, have been shown previously to impair the transcriptional activity of the protein but not its ability to support viral DNA replication. To understand the biochemical basis of this defect, we have used the TADs of a low-risk (HPV11) and a high-risk (HPV31) human… CONTINUE READING