Amino acid sequence of the tryptic peptide containing the alkylamine-reactive site from human alpha 2-macroglobulin. Identification of gamma-glutamylmethylamide.

@article{Swenson1980AminoAS,
  title={Amino acid sequence of the tryptic peptide containing the alkylamine-reactive site from human alpha 2-macroglobulin. Identification of gamma-glutamylmethylamide.},
  author={Richard P Swenson and James W. Howard},
  journal={The Journal of biological chemistry},
  year={1980},
  volume={255 17},
  pages={8087-91}
}
Human alpha 2-macroglobulin (alpha 2M) is inhibited by covalent reaction with alkylamines. The site of methylamine incorporation has been proposed to be an activated glutamyl residue (Swenson, R. P., and Howard, J. B. (1979) Proc. Natl. Acad. Sci. U. S. A. 76, 4313-4316). A large, 56-amino acid residue glycopeptide derived from tryptic cleavage of [14C]methylamine-labeled alpha 2M was isolated. Based upon recovery of the specific radioactivity in the peptide, there appears to be only a single… CONTINUE READING