Amino acid sequence of rabbit fast-twitch skeletal muscle calsequestrin deduced from cDNA and peptide sequencing ( calsequestrin / sarcoplasmic reticulum / cDNA cloning )

@inproceedings{FLIEGELAminoAS,
  title={Amino acid sequence of rabbit fast-twitch skeletal muscle calsequestrin deduced from cDNA and peptide sequencing ( calsequestrin / sarcoplasmic reticulum / cDNA cloning )},
  author={LARRY FLIEGEL and MAMORU OHNISHIt and MICHAEL R. CARPENTERt and VIJAY K. KHANNA and F. REINHARTA. and REITHMEIERt and David MacLennan}
}
Partial amino acid sequence analysis of rabbit fast-twitch skeletal muscle calsequestrin permitted the construction of synthetic oligonucleotides that were used as both primers and probes for the synthesis and isolation of cDNAs encoding calsequestrin from neonatal rabbit skeletal muscle libraries. The cDNA sequence encodes a processed protein of 367 residues with a Mr of 42,435 and a 28-residue aminoterminal signal sequence. The deduced amino acid sequence agreed closely with the portions of… CONTINUE READING