Amino acid sequence of a coagulant enzyme, flavoxobin, from Trimeresurus flavoviridis venom.

@article{Shieh1988AminoAS,
  title={Amino acid sequence of a coagulant enzyme, flavoxobin, from Trimeresurus flavoviridis venom.},
  author={Ted C Shieh and Shun-ichiro Kawabata and Hiroshi Kihara and Motonori Ohno and Sadaaki Iwanaga},
  journal={Journal of biochemistry},
  year={1988},
  volume={103 4},
  pages={596-605}
}
The amino acid sequence of a coagulant enzyme, named flavoxobin, isolated from the venom of Trimeresurus flavoviridis (the habu snake) was determined by sequencing the S-pyridylethylated derivative of the protein and its peptides generated by chemical (cyanogen bromide and hydroxylamine) and enzymatic (clostripain, Staphylococcus aureus V8 protease, Achromobacter protease I, and elastase) cleavages. Hydrazinolysis was also employed to determine the C-terminal amino acid. The enzyme consisted of… CONTINUE READING

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