Amino acid sequence analysis, gene construction, cloning, and expression of gelonin, a toxin derived from Gelonium multiflorum.

@article{Rosenblum1995AminoAS,
  title={Amino acid sequence analysis, gene construction, cloning, and expression of gelonin, a toxin derived from Gelonium multiflorum.},
  author={Michael G. Rosenblum and W A Kohr and Kenneth Loren Beattie and Wanda G. Beattie and Willy Marks and Peter Toman and Linda W Cheung},
  journal={Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research},
  year={1995},
  volume={15 6},
  pages={547-55}
}
The plant toxin gelonin is an extremely potent inhibitor of protein synthesis, similar in action to ricin. The mature protein primary sequence was obtained using conventional sequencing techniques. Gelonin was found to be composed of 258 amino acids and contains 21 lysine residues. This toxin shares approximately 33% sequence homology with trichosanthin and ricin A chain. A 774 bp synthetic gene encoding gelonin was synthesized and expressed in E. coli. Recombinant gelonin (approximately 28 kD… CONTINUE READING

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