Amino acid replacements at the H2-activating site of the NAD-reducing hydrogenase from Alcaligenes eutrophus.

@article{Massanz1999AminoAR,
  title={Amino acid replacements at the H2-activating site of the NAD-reducing hydrogenase from Alcaligenes eutrophus.},
  author={C Massanz and B. Cornelia Friedrich},
  journal={Biochemistry},
  year={1999},
  volume={38 43},
  pages={14330-7}
}
The role of amino acid residues in the H(2)-activating subunit (HoxH) of the NAD-reducing hydrogenase (SH) from Alcaligenes eutrophus has been investigated by site-directed mutagenesis. Conserved residues in the N-terminal L1 (RGxE) and L2 (RxCGxCx(3)H) and the C-terminal L5 (DPCx(2)Cx(2)H/R) motifs of the active site-harboring subunit were chosen as targets. Crystal structure analysis of the [NiFe] hydrogenase from Desulfovibrio gigas uncovered two pairs of cysteines (motifs L2 and L5) as… CONTINUE READING