Amino acid positions 69-132 of UGT1A9 are involved in the C-glucuronidation of phenylbutazone.

@article{Nishiyama2008AminoAP,
  title={Amino acid positions 69-132 of UGT1A9 are involved in the C-glucuronidation of phenylbutazone.},
  author={Takahito Nishiyama and Miki Fujishima and Yasuhiro Masuda and Tadashi Izawa and Tomokazu Ohnuma and Keiichi Ogura and Atsushi Hiratsuka},
  journal={Archives of biochemistry and biophysics},
  year={2008},
  volume={478 1},
  pages={
          75-80
        }
}
Phenylbutazone (PB) is known to be biotransformed to its O- and C-glucuronide. Recently, we reported that PB C-glucuronide formation is catalyzed by UGT1A9. Interestingly, despite UGT1A8 sharing high amino acid sequence identity with UGT1A9, UGT1A8 had no PB C-glucuronidating activity. In the present study, we constructed eight UGT1A9/UGT1A8 chimeras and evaluated which region is important for PB C-glucuronide formation. All of the chimeras and UGT1A8 and UGT1A9 had 7-hydroxy-(4-trifluoromethyl… CONTINUE READING