Amino acid activation with adenosine 5′-phosphorimidazolide

@article{Weber1978AminoAA,
  title={Amino acid activation with adenosine 5′-phosphorimidazolide},
  author={Arthur L. Weber and Leslie E. Orgel},
  journal={Journal of Molecular Evolution},
  year={1978},
  volume={11},
  pages={9-16}
}
Amino acids are activated by reaction with adenosine 5′-phosphorimidazolide in aqueous imidazole buffers. If adenosine 5′-(O-methylphosphate), an analogue of the 3′-terminus of t-RNA is present, 2′(3′)-O-aminoacyladenosine 5′-(O-methylphosphate) is formed. Fifteen percent of this compound accumulated at pH 5.8, but less was formed at higher pHs. The highest efficiency of utilization of ImpA attained in our experiments was about 24%. Analogous reactions occured with several other amino acids… CONTINUE READING