Amino Acids that Confer Transport of Raffinose and Maltose Sugars in the Raffinose Permease (RafB) of Escherichia coli as Implicated by Spontaneous Mutations at Val-35, Ser-138, Ser-139, Gly-389 and Ile-391

@article{Camp2007AminoAT,
  title={Amino Acids that Confer Transport of Raffinose and Maltose Sugars in the Raffinose Permease (RafB) of Escherichia coli as Implicated by Spontaneous Mutations at Val-35, Ser-138, Ser-139, Gly-389 and Ile-391},
  author={Bonnie M. Van Camp and Robert R. Crow and Y. Peng and M. F. Varela},
  journal={Journal of Membrane Biology},
  year={2007},
  volume={220},
  pages={87-95}
}
  • Bonnie M. Van Camp, Robert R. Crow, +1 author M. F. Varela
  • Published 2007
  • Biology, Medicine
  • Journal of Membrane Biology
  • In order to identify amino acid residues in the Escherichia coli raffinose-H+ permease (RafB) that play a role in sugar selection and transport, we first incubated E. coli HS4006 containing plasmid pRU600 (expresses inducible raffinose permease and α-galactosidase) on maltose MacConkey indicator plates overnight. Initially, all colonies were white, indicating no fermentation of maltose. Upon further incubation, 100 mutants appeared red. pRU600 DNA was prepared from 55 mutants. Five mutants… CONTINUE READING
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