Amine-reactive isobaric tagging reagents: requirements for absolute quantification of proteins and peptides.

@article{Quaglia2008AminereactiveIT,
  title={Amine-reactive isobaric tagging reagents: requirements for absolute quantification of proteins and peptides.},
  author={Milena Quaglia and Caroline Pritchard and Zoe Hall and Gavin O'Connor},
  journal={Analytical biochemistry},
  year={2008},
  volume={379 2},
  pages={164-9}
}
Amine-reactive isobaric tagging reagents such as iTRAQ (isobaric tags for relative and absolute quantitation) have recently become increasing popular for relative protein quantification, cell expression profiling, and biomarker discovery. This is due mainly to the possibility of simultaneously identifying and quantifying multiple samples. The principles of iTRAQ may also be applied to absolute protein quantification with the use of synthetic peptides as standards. The prerequisites that must be… CONTINUE READING

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C. Arsene, R. Ohlendorf, W. Burkitt, C. Pritchard, A. Hernion
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Protein quantification by isotope dilution mass spectrometry of proteolytic fragments : Cleavage rate and precision

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