Amine coupling through EDC/NHS: a practical approach.

@article{Fischer2010AmineCT,
  title={Amine coupling through EDC/NHS: a practical approach.},
  author={Marcel J. E. Fischer},
  journal={Methods in molecular biology},
  year={2010},
  volume={627},
  pages={
          55-73
        }
}
Surface plasmon resonance (SPR) is one of the leading tools in biomedical research. The challenge in its use is the controlled positioning of one of the components of an interaction on a carefully designed surface. Many attempts in interaction analysis fail due to the non-functional or unsuccessful immobilization of a reactant onto the complex matrix of that surface. The most common technique for linking ligands covalently to a hydrophilic solid surface is amine coupling via reactive esters. In… CONTINUE READING
BETA

Similar Papers

Figures, Tables, and Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 76 CITATIONS, ESTIMATED 64% COVERAGE

Approaches for Conjugating Tailor-Made Polymers to Proteins.

  • Methods in enzymology
  • 2017
VIEW 3 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED

DEVELOPMENT OF MP-SPR BIOSENSOR ANALYSIS METHOLOGY

Ph. D. Niko Granqvist
  • 2017
VIEW 2 EXCERPTS
CITES METHODS
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

2011
2019

CITATION STATISTICS

  • 9 Highly Influenced Citations

  • Averaged 10 Citations per year from 2017 through 2019

References

Publications referenced by this paper.
SHOWING 1-8 OF 8 REFERENCES

Determination by surface plasmon resonance of the binding characteristics of a kinase and its substrates

N. J. de Mol, M.J.E. Fischer, J. Castrop, T. Frelink
  • Biomol. Interactions
  • 2006
VIEW 9 EXCERPTS
HIGHLY INFLUENTIAL

Label-free screening of bio-molecular interactions

  • Analytical and bioanalytical chemistry
  • 2003
VIEW 2 EXCERPTS

Kinetic analysis of the mass transport limited interaction between the tyrosine kinase lck SH2 domain and a phosphorylated peptide studied by a new cuvette based surface Plasmon resonance instrument

N. J. de Mol, E. Plomp, M.J.E. Fischer, R. Ruijtenbeek
  • Anal. Biochem
  • 2000
VIEW 2 EXCERPTS

Improving biosensor analysis.

  • Journal of molecular recognition : JMR
  • 1999
VIEW 1 EXCERPT