Amide proton back-exchange in deuterated peptides: applications to MS and NMR analyses.

@article{Feng2006AmidePB,
  title={Amide proton back-exchange in deuterated peptides: applications to MS and NMR analyses.},
  author={Lianmei Feng and Ron Orlando and James H. Prestegard},
  journal={Analytical chemistry},
  year={2006},
  volume={78 19},
  pages={6885-92}
}
Deuterium for hydrogen exchange at amide sites in proteins is a well-established means of probing the stability of certain proteins and the effects of interactions with ligands and other proteins. When deuterium content is analyzed by mass spectrometry (MS) of digested peptides, corrections frequently need to be made for back-exchange that occurs during digestion, separation, and analysis. The back-exchange process is actually complex and deserving of analysis in a sequence-specific manner… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…