Amelioration of streptozotocin-induced diabetes in mice using human islet cells derived from long-term culture in vitro.

@article{Zhao2002AmeliorationOS,
  title={Amelioration of streptozotocin-induced diabetes in mice using human islet cells derived from long-term culture in vitro.},
  author={Min Zhao and Michael R. Christie and Nigel Heaton and Sarah George and Stephanie Amiel and Guo Cai Huang},
  journal={Transplantation},
  year={2002},
  volume={73 9},
  pages={1454-60}
}
BACKGROUND Long-term maintenance of the phenotype of beta cells in vitro is difficult. The objective of this study was to examine an in vitro method for preserving the capacity of adult human beta cells to express insulin. We evaluated the use of long-term cultured islet cells for the treatment of diabetic SCID mice. METHODS Human islets were isolated from cadaveric donors. The islets were cultured as monolayers and clusters in repeating cycles for 4 months. Thereafter, the cells were tested… CONTINUE READING
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