Amelioration of cataracts and proteolysis in cultured lenses by cysteine protease inhibitor E64.

@article{Shearer1991AmeliorationOC,
  title={Amelioration of cataracts and proteolysis in cultured lenses by cysteine protease inhibitor E64.},
  author={Thomas R. Shearer and Mitsuyoshi Azuma and Larry L. David and Takashi Murachi},
  journal={Investigative ophthalmology & visual science},
  year={1991},
  volume={32 3},
  pages={533-40}
}
Cataracts were produced in cultured rat lenses by either 10 microM calcium ionophore A23187, 25 microM sodium selenite, or 30 mM xylose. E64, an inhibitor of cysteine proteases, such as calpain (EC, 3.4.22.17), reduced severity of cataract and proteolysis of crystallins when included at a 500 microM concentration in the culture medium along with cataractogenic agents. Calpain II enzyme activity and the amount of calpain antigen were decreased in the cytosol of cataractous lens. However, E64… CONTINUE READING

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