Amber, ochre and opal suppressor tRNA genes derived from a human serine tRNA gene.

@article{Capone1985AmberOA,
  title={Amber, ochre and opal suppressor tRNA genes derived from a human serine tRNA gene.},
  author={John P. Capone and Phillip A. Sharp and Uttam L. RajBhandary},
  journal={The EMBO journal},
  year={1985},
  volume={4 1},
  pages={213-21}
}
Amber, ochre and opal suppressor tRNA genes have been generated by using oligonucleotide directed site-specific mutagenesis to change one or two nucleotides in a human serine tRNA gene. The amber and ochre suppressor (Su+) tRNA genes are efficiently expressed in CV-1 cells when introduced as part of a SV40 recombinant. The expressed amber and ochre Su+ tRNAs are functional as suppressors as demonstrated by readthrough of the amber codon which terminates the NS1 gene of an influenza virus or the… CONTINUE READING

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