• DOI:10.1038/sj.onc.1201691
• Corpus ID: 34508962

Alternatively spliced forms of cyclin D1 modulate entry into the cell cycle in an inverse manner

@article{Sawa1998AlternativelySF,
  title={Alternatively spliced forms of cyclin D1 modulate entry into the cell cycle in an inverse manner},
  author={Hiroki Sawa and Teruyo Arato Ohshima and Hiroyuki Ukita and Hiromi Murakami and Yukie Chiba and Hajime Kamada and Mitsuhiro Hara and Isamu Saito},
  journal={Oncogene},
  year={1998},
  volume={16},
  pages={1701-1712}
}

• H. Sawa, Teruyo Arato Ohshima, +5 authors I. Saito
• Published 2 April 1998
• Biology
• Oncogene

Alternative splicing of cyclin D1 gene mRNA has recently been demonstrated. The novel transcript shows no splicing at the downstream exon 4 boundary and encodes a protein with an altered carboxyl-terminal domain that is a cyclin D1 variant; exon 5 is not included in the coding sequence which terminates downstream of exon 4. We here produced cells that exogenously express each form of cyclin D1 and analysed their cell cycle regulation. We found that (1) alternative splicing forms of cyclin D1 modulated entry into the cell cycle in an inverse manner; (2) both splicing forms suppressed cell growth; and (3) cells overexpressing form [a] were inhibited from entry into and completion of the S phase, although form [b]-expressing cells showed no reduction of G1- to S transition. We also found that overexpression of either cyclin D1 form up-regulated Rb gene products, suggesting that this up-regulation may be one of the causes of growth suppression in cyclin D1 overexpressing cells.