Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.

@article{Pl2005AlternativeVO,
  title={Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.},
  author={G{\'a}bor P{\'a}l and Shun-Yin Fong and A. Kossiakoff and Sachdev S. Sidhu},
  journal={Protein science : a publication of the Protein Society},
  year={2005},
  volume={14 9},
  pages={2405-13}
}
Combinatorial shotgun scanning mutagenesis was used to analyze two large, related protein binding sites to assess the specificity and importance of individual side chain contributions to binding affinity. The strategy allowed for cost-effective generation of a plethora of functional data. The ease of the technology promoted comprehensive investigations, in which the classic alanine-scanning approach was expanded with two additional strategies, serine- and homolog-scanning. Binding of human… CONTINUE READING