Alternative pathways of human islet amyloid polypeptide aggregation distinguished by (19)f nuclear magnetic resonance-detected kinetics of monomer consumption.

@article{Suzuki2012AlternativePO,
  title={Alternative pathways of human islet amyloid polypeptide aggregation distinguished by (19)f nuclear magnetic resonance-detected kinetics of monomer consumption.},
  author={Yuta Suzuki and Jeffrey R. Brender and Kevin Hartman and Ayyalusamy Ramamoorthy and E Neil G Marsh},
  journal={Biochemistry},
  year={2012},
  volume={51 41},
  pages={8154-62}
}
Amyloid formation, a complex process involving many intermediate states, is proposed to be the driving force for amyloid-related toxicity in common degenerative diseases. Unfortunately, the details of this process have been obscured by the limitations in the methods that can follow this reaction in real time. We show that alternative pathways of aggregation can be distinguished by using (19)F nuclear magnetic resonance (NMR) to monitor monomer consumption along with complementary measurements… CONTINUE READING