Alternative oxidase in durum wheat mitochondria. Activation by pyruvate, hydroxypyruvate and glyoxylate and physiological role.

@article{Pastore2001AlternativeOI,
  title={Alternative oxidase in durum wheat mitochondria. Activation by pyruvate, hydroxypyruvate and glyoxylate and physiological role.},
  author={Donato Pastore and Daniela Trono and Maura Nicoletta Laus and Natale Di Fonzo and Salvatore Passarella},
  journal={Plant & cell physiology},
  year={2001},
  volume={42 12},
  pages={1373-82}
}
In order to gain a first insight into the alternative oxidase (AO) function in durum wheat mitochondria (DWM), we investigated some activation pathways of this enzyme in DWM purified from both etiolated shoots and green leaves. AO was activated when DWM were added with either pyruvate, known as an AO activator in other plant mitochondria, or alanine plus 2-oxoglutarate, which can generate intramitochondrial pyruvate and glutamate via transamination. In contrast, no AO activity was observed… CONTINUE READING
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