Alternative mechanisms of transcriptional activation by Rap1p.

  title={Alternative mechanisms of transcriptional activation by Rap1p.},
  author={F Z Idrissi and Nat{\`a}lia Garcia-Reyero and Juan Bautista Fern{\'a}ndez-Larrea and Benjam{\'i}n Pi{\~n}a},
  journal={The Journal of biological chemistry},
  volume={276 28},
Single Rap1p DNA-binding sites are poor activators of transcription of yeast minimal promoters, even when fully occupied in vivo. This low efficiency is due to two independent repression mechanisms as follows: one that requires the presence of histones, and one that requires Hrs1p, a component of the RNA polymerase II mediator complex. Both repression mechanisms were greatly reduced for constructs with tandemly arranged sites. In these constructs, UASrpg sequences (ACACCCATACATTT) activated… CONTINUE READING