Macrophage infiltration and activation in metabolic tissues underlie obesity-induced insulin resistance and type 2 diabetes. While inflammatory activation of resident hepatic macrophages potentiates insulin resistance, the functions of alternatively activated Kupffer cells in metabolic disease remain unknown. Here we show that in response to the Th2… (More)
Figure 3. Conjugated Oleic Acid Potentiates Alternative Macrophage Activation (A) Oleic acid potentiates IL-4 induction of arginase activity in wild-type, but not PPARd / , BMDMs. (B–E) PPARd is required for potentiation of alternative activation by oleic acid. BMDMs from 129/SvJ wild-type and PPARd / mice were stimulated with IL-4 for 48 hr in the presence of BSA or BSA:oleic acid conjugate. qRT-PCR analyses were performed on mRNAs for Arg1 (B), Clec7a (dectin-1) (C), Pdcd1lg2 (D), and Chi3l3 (E). Data are presented as mean ± SEM. *p < 0.05; **p < 0.01.