Alternate electron acceptors for medium-chain acyl-CoA dehydrogenase: use of ferricenium salts.

@article{Lehman1990AlternateEA,
  title={Alternate electron acceptors for medium-chain acyl-CoA dehydrogenase: use of ferricenium salts.},
  author={Tara Lehman and Colin Thorpe},
  journal={Biochemistry},
  year={1990},
  volume={29 47},
  pages={10594-602}
}
Medium-chain acyl-CoA dehydrogenase reduced with octanoyl-CoA is reoxidized in two one-electron steps by two molecules of the physiological oxidant, electron transferring flavoprotein (ETF). The organometallic oxidant ferricenium hexafluorophosphate (Fc+PF6-) is an excellent alternative oxidant of the dehydrogenase and mimics a number of the features shown by ETF. Reoxidation of octanoyl-CoA-reduced enzyme (200 microM Fc+PF6- in 100 mM Hepes buffer, pH 7.6, 1 degree C) occurs in two one… CONTINUE READING
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