Altered molecular species of human interferon produced in the presence of inhibitors of glycosylation.

@article{Havell1977AlteredMS,
  title={Altered molecular species of human interferon produced in the presence of inhibitors of glycosylation.},
  author={E A Havell and Shudo Yamazaki and Jan T. Vil{\vc}ek},
  journal={The Journal of biological chemistry},
  year={1977},
  volume={252 12},
  pages={
          4425-7
        }
}
The inhibitors of glycosylation, 2-deoxy-D-glucose or D-glucosamine, inhibit the synthesis of biologically active interferon in human FS-4 fibroblast cultures stimulated with polyinosinate-polycytidylate. Interferon synthesized in the presence of partially inhibitory concentrations of 2-deoxy-D-glucose or D-glucosamine were found to differ from interferons made in control cultures in some physical properties. Interferons synthesized in the presence of either inhibitor had a diminished charge… Expand
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References

SHOWING 1-10 OF 16 REFERENCES
Suppression of human interferon production by inhibitors of glycosylation.
TLDR
The results are compatible with the idea that the two inhibitors suppress the production of biologically active interferon by virtue of their interfering with the proper glycosylation of the molecule. Expand
Apparent dispensability of the carbohydrate moiety of human interferon for antiviral activity.
TLDR
Human leukocyte and tritium-labeled fibroblast interferons, prepared by induction with Sendai virus and with double-stranded polyinosinic acid respectively, have been studied in relation to the carbohydrate moieties attached to them to reduce the heterogeneous character of interferon. Expand
Intracellular location of newly synthesized interferon in human FS-4 cells.
TLDR
It is concluded that probably all interferon in this cell system is synthesized on membrane-bound polysomes, discharged into the lumina of the rough endoplasmic reticulum, and further processed during passage through various intracellular membrane compartments. Expand
Interferon: evidence for its glycoprotein nature.
  • F. Dorner, M. Scriba, R. Weil
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1973
TLDR
It is concluded that rabbit interferon is a glycoprotein containing the terminal oligosaccharide sequence sialic acid --> galactose, which contains both (3)H and (14)C activity. Expand
Molecular heterogeneity of human leukocyte interferon: two populations differing in molecular weights, requirements for renaturation, and cross-species antiviral activity.
TLDR
It is reported that human leukocyte interferon preparations do, indeed, contain two molecular populations that can be distinguished physically, chemically, and biologically. Expand
ANTIGENIC, PHYSICOCHEMICAL, AND BIOLOGIC CHARACTERIZATION OF HUMAN INTERFERONS *
TLDR
It was shown that human interferons made in cultures of buffy coat cells and in diploid cell strains (“fibroblast” interferon) are dissimilar in many respects and sera made against fibroblast interferOn, while neutralizing the activity of homologous interfer on, completely failed to neutralize leukocyte interfer~n. Expand
Production of High-Titered Interferon in Cultures of Human Diploid Cells
TLDR
Under the optimal conditions carefully defined in the authors' experiments, superinduction produced about a 100-fold increase over the average control yield, resulting in interferon yields of about 10,000 reference units from cultures containing about 106 cells, which were similar to that obtained in cultures of human embryonic kidney cells and in FS-3 cells stimulated with other double-stranded polynucleotide inducers. Expand
Molecular structure of human fibroblast and leukocyte interferons: probe by lectin and hydrophobic chromatography
TLDR
The results suggest that at least subtle differences in the processing of the induction signal (virus or rIn-rCn) within the same cell type may occur, slightly altering some structural features. Expand
Interferon: purification and initial characterization from human diploid cells.
  • E. Knight
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1976
TLDR
Oxidation of this polypeptide with periodic acid and subsequent staining with Fuchsin base indicates that it contains carbohydrate ans suggests that the human fibroblast interferon is a glycoprotein. Expand
Inhibition of the multiplication of enveloped viruses by glucose derivatives.
  • C. Scholtissek
  • Chemistry, Medicine
  • Current topics in microbiology and immunology
  • 1975
TLDR
Two groups independently found that with glucose derivatives like 2-deoxy-D-glucose or glucosamine, the synthesis of influenza glycoproteins was specifically inhibited, while the yield of carbohydrate-free viral proteins was not significantly affected. Expand
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