Altered growth of human colon cancer cell lines disrupted at activated Ki-ras.

@article{Shirasawa1993AlteredGO,
  title={Altered growth of human colon cancer cell lines disrupted at activated Ki-ras.},
  author={Seiji Shirasawa and Masanori Furuse and Noriko Yokoyama and Takehiko Sasazuki},
  journal={Science},
  year={1993},
  volume={260 5104},
  pages={
          85-8
        }
}
Point mutations that activate the Ki-ras proto-oncogene are presented in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and… 
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