Altered flexibility in the substrate-binding site of related native and engineered high-alkaline Bacillus subtilisins.

@article{Mulder1999AlteredFI,
  title={Altered flexibility in the substrate-binding site of related native and engineered high-alkaline Bacillus subtilisins.},
  author={Frans A. A. Mulder and Dick Schipper and Richard R Bott and Rolf Boelens},
  journal={Journal of molecular biology},
  year={1999},
  volume={292 1},
  pages={111-23}
}
High-alkaline serine proteases have been successfully applied as protein degrading components of detergent formulations and are subject to extensive protein engineering efforts to improve their stability and performance. Dynamics has been suggested to play an important role in determining enzyme activity and specificity and it is therefore of interest to establish how local changes in internal mobility affect protein stability, specificity and performance. Here we present the dynamic properties… CONTINUE READING