Altered expression of ubiquitous kinesin heavy chain results in resistance to etoposide and hypersensitivity to colchicine: mapping of the domain associated with drug response.

@article{Axenovich1998AlteredEO,
  title={Altered expression of ubiquitous kinesin heavy chain results in resistance to etoposide and hypersensitivity to colchicine: mapping of the domain associated with drug response.},
  author={Sergey A. Axenovich and Alexander R. Kazarov and Alexander D. Boiko and Gabriella Armin and Igor B. Roninson and Andrei V. Gudkov},
  journal={Cancer research},
  year={1998},
  volume={58 15},
  pages={3423-8}
}
The motor protein kinesin is a tetramer consisting of two heavy and two light chains. Expression of an antisense RNA fragment derived from the mouse ubiquitous kinesin heavy chain (uKHC) cDNA is associated with a unique type of multidrug resistance. We analyzed the effects of retroviral transduction of the human uKHC and its derivatives on drug sensitivity of the human fibrosarcoma cell line HT1080. Surprisingly, overexpression of full-length uKHC and its variants that were deficient in the NH2… CONTINUE READING