Altered expression of the calcitonin gene associated with RNA polymorphism

@article{Rosenfeld1981AlteredEO,
  title={Altered expression of the calcitonin gene associated with RNA polymorphism},
  author={Michael G. Rosenfeld and Susan G. Amara and Bernard A. Roos and Estelita Sebastian Ong and Ronald M. Evans},
  journal={Nature},
  year={1981},
  volume={290},
  pages={63-65}
}
The selective control of gene expression results in diversified morphology and physiological function. Understanding the expression of differentiated function in molecular terms requires detailed characterization of the regulation of mRNA synthesis and catabolism. Although considerable emphasis has been placed on transcriptional control, the discovery of HnRNA and ‘split genes’ gives rise to the possibility of post-transcriptional regulation at the level of processing of nuclear precursors1,2… 
Calcitonin mRNA polymorphism: P alternative RNA splicing events (RNA processing regulation/hormone biosynthesis/functional domai
Evidence is presented which supports a model proposing that differential RNA splicing events may be used in expression of genes of the endocrine system to generate alternative polypeptide hormones.
Expression and function of the calcitonin gene products.
TLDR
Although the cloning of the calcitonin genes has led to the discovery of more new and interesting information than almost any other similar gene, there are good reasons to believe that there is more to come.
Calcitonin Gene—Related Peptide: A Neuropeptide Generated as a Consequence of Tissue‐Specific, Developmentally Regulated Alternative RNA Processing Events
TLDR
The distribution of C GRP mRNA and peptide in the brain and other tissues and initial studies of biological actions of the synthetic peptide suggest functions for CGRP at the neuromuscular junction in modulation of cardiovascular homeostasis5 and in pain perception.
Expression of the Calcitonin Gene Family in Medullary Thyroid Carcinoma
TLDR
The methods employed for detection of gene expression and for measurement and characterized of peptide products are described, and the relevance of biochemical tumor markers is discussed in relation to the new diagnostic methods for inherited MTC based on molecular biological techniques.
Control of calcitonin/calcitonin gene-related peptide pre-mRNA processing by constitutive intron and exon elements
The calcitonin/calcitonin gene-related peptide (CGRP) primary transcript is alternatively spliced in thyroid C cells and neurons, resulting in the tissue-specific production of calcitonin and CGRP
Calcitonin/calcitonin gene-related peptide transcription unit: tissue-specific expression involves selective use of alternative polyadenylation sites
Different 3' coding exons in the rat calcitonin gene are used to generate distinct mRNAs encoding either the hormone calcitonin in thyroidal C-cells or a new neuropeptide referred to as calcitonin
Expression of the first calcitonin/CGRP gene in spontaneous and transplanted rat medullary thyroid carcinoma: a comparison of dot-blot analysis, in situ hybridization, and immunohistochemistry.
TLDR
RISH proved to be as sensitive as dot-blot analysis to detect gene expression in tissue samples and showed that each technique is equal in value and that they are complementary to each other.
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References

SHOWING 1-10 OF 12 REFERENCES
Characterization of rat calcitonin mRNA.
TLDR
A chimeric plasmic containing cDNA complementary to rat calcitonin mRNA has been constructed and partial sequence analysis shows that the insert contains a nucleotide sequence encoding the complete amino acid sequence of calciton in a manner analogous to that of other small polypeptide hormones.
Identification of the putative cell-free translation product of rat calcitonin mRNA.
TLDR
Immunoprecipitations of the cell-free translation products demonstrate that the 17,500-dalton protein has immunologic determinants which are specifically recognized by antisera to calcitonin.
Transcription units for mRNA production in eukaryotic cells and their DNA viruses.
  • J. Darnell
  • Biology, Medicine
    Progress in nucleic acid research and molecular biology
  • 1979
Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells.
TLDR
It is demonstrated that rMTC 6-23 cells contain NT and the possibility of an association between neurotensin and calcitonin is suggested, and the presence of a tridecapeptide originally isolated from the hypothalamus and not previously associated with C cells is suggested.
Tumor growth and calcitonin during serial transplantation of rat medullary thyroid carcinoma.
TLDR
Using serial plasma CT analyses, this work propagated 16 transplantable series of medullary thyroid carcinoma (MTC) from 2 types of WAG/Rij rat MTC from The Netherlands to facilitate propagation of MTC series needed for particular studies of CT production and cancer.
Ectopic (inappropriate) hormone production by tumors: mechanisms involved and the biological and clinical implications.
TLDR
This work has shown that a wide range of endocrine tumors secrete hormones not normally associated with the gland in which the neoplasm arises and that nonendocrine tumors can synthesize and/or secrete certain polypeptide hormones and other “tumor-associated” proteins.
Cell-free synthesis of fish preproinsulin, and processing by heterologous mammalian microsomal membranes.
  • D. Shields, G. Blobel
  • Medicine, Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1977
Poly(A)-containing mRNA isolated from the islets of Langerhans obtained from two species of fish, angler fish (Lophius americanus) and sea raven (Hemitripterus americanus), stimulated protein
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
  • G. Wahl, M. Stern, G. Stark
  • Chemistry, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1979
TLDR
Ten percent dextran sulfate accelerates the rate of hybridization of randomly cleaved double-stranded DNA probes to immobilized nucleic acids by as much as 100-fold, without increasing the background significantly.
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
  • G. Mcmaster, G. Carmichael
  • Chemistry, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1977
TLDR
A simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis, using the metachromatic stain acridine orange for visualization ofucleic acids in gels and which can be analyzed on the same slab gel.
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
  • J. Alwine, D. Kemp, G. Stark
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1977
TLDR
This procedure allows detection of specific RNA bands with high sensitivity and low background by hybridization with 32P-labeled DNA probes followed by autoradiography.
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