Altered epidermal growth factor (EGF)-stimulated protein kinase activity in variant A431 cells with altered growth responses to EGF.

@article{Buss1982AlteredEG,
  title={Altered epidermal growth factor (EGF)-stimulated protein kinase activity in variant A431 cells with altered growth responses to EGF.},
  author={Janice E. Buss and Jeffrey E. Kudlow and Cheri S. Lazar and Gordon N. Gill},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 8},
  pages={
          2574-8
        }
}
To determine the role of epidermal growth factor (EGF)-stimulated protein kinase in the biological effects caused by EGF, tyrosine-specific kinase activity has been quantitated in A431 human epidermoid carcinoma cells and six variant cell lines. Because EGF inhibited proliferation of A431 cells, variants resistant to this inhibition were selected by treatment with mutagen and maintenance for 1 month in 0.1 muM EGF. After cloning and growth for 6-20 generations without EGF, the resistance of the… CONTINUE READING

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