Corpus ID: 2942112

Altered distribution and excretion of N-1-methylnicotinamide in rats with Walker 256 carcinosarcoma.

  title={Altered distribution and excretion of N-1-methylnicotinamide in rats with Walker 256 carcinosarcoma.},
  author={B. Clark and J. Murai and A. Pomeranz and P. Mills and R. Halpern},
  journal={Cancer research},
  volume={35 7},
Levels of nicotinamide and N-1-methylnicotinamide in serum, liver, and kidney as well as renal clearances and 24-hr urine levels of N-1-methylnicotinamide were compared in normal rats and rats bearing Walker 256 tumors. There was no significant difference between normal and tumor-bearing rats with regard to nicotinamide levels. With regard to N-1-methylnicotinamide, tumor-bearing rats had significantly lower serum and liver levels and significantly higher 24-hr urine levels and renal clearances… Expand
Methylation of nicotinamide in rat liver cytosol and its correlation with hepatocellular proliferation.
Results support the view that nicotinamide methylase and its product, 1-methyl-nicotinamide, are involved in the control of hepatocellular DNA synthesis and proliferation. Expand
N1‐Methylnicotinamide Level in the Blood after Nicotinamide Loading as Further Evidence for Malignant Tumor Burden
Loading of Nmd (500 mg/kg body weight) was closely correlated with this enzyme activity in the liver from the early to the terminal stage of tumor development, and similar correlations were seen in the animal groups bearing various other tumors, although almost all of the animals bearing these tumors showed a higher enzyme activity than their control normal animals. Expand
Nicotinamide methylation. Tissue distribution, developmental and neoplastic changes.
Rat liver exhibited the highest nicotinamide methylase activity among all adult tissues tested; other rat tissues, like adrenal, pancreas, kidney, brain and mouse tissues, had only less than 15% of the adult rat liver activity. Expand
1-methylnicotinamide and NAD metabolism in normal and transformed normal rat kidney cells.
No evidence was obtained that its synthesis is involved with maintenance of nicotinamide of S-adenosylmethionine levels, and the biological function for 1-methylnicotinamide remains a mystery. Expand
1-Methylnicotinamide metabolism in cultured cells
1-Methylnicotinamide is a major urinary metabolite in mammals (Huff and Perlzweig, 1943). Its structure arises via a methyl transfer from S-adenosylmethionine (AdoMet) to the ring nitrogen ofExpand
Metabolism of NAD and N1-methylnicotinamide in growing and growth-arrested cells.
  • G. S. Johnson
  • Chemistry, Medicine
  • European journal of biochemistry
  • 1980
The results demonstrate a rapid turnover of NAD at the bond joining nicotinamide and ADP-ribose, in agreement with previous studies, and show that Nicotinamide is metabolized into N1-methylnicotinamide with what appears to be a carefully regulated synthetic mechanism. Expand
Increased urinary excretion of 1-methyl-2-pyridone-5-carboxamide in rats administered 2-acetylaminofluorene.
During the administration of the hepatocarcinogen, 2-acetylaminofluorene (AAF), to Wistar strain male rats in the diet excretion of 1-methyl-2-pyridone-5-carboxamide (2-PY) gradually increased, and this increased level was maintained even after the treatment had been terminated. Expand
1-Methylnicotinamide stimulates cell growth and inhibits hemoglobin synthesis in differentiating murine erythroleukemia cells.
It appears that 1-MN has opposing effects on growth and induction of differentiation than those seen in MELC cultures exposed to NA or N'-MN. Expand
Nicotinamide methyltransferase activity and cell-death in the liver of the mouse bearing Ehrlich ascites tumor.
In order to develop new ways of tumor diagnosis and therapy, it is important to know the metabolic changes occurring while bearing the tumor, since these ways should be applied to the cancer patient,Expand
Metabolomic Analysis of Liver Tissue from the VX2 Rabbit Model of Secondary Liver Tumors
Normal liver tissue harboring malignancy had a distinct metabolic signature and the role of metabolic profiles on liver biopsies for the detection of early liver cancer remains to be determined. Expand


Renal cortical slice uptake and runout of N-methylnicotinamide and p-aminohippurate after potassium dichromate treatment.
  • G. Hirsch, A. Pakuts
  • Chemistry, Medicine
  • Canadian journal of physiology and pharmacology
  • 1974
Administration of 40 mg/kg potassium dichromate to adult male rats produced a significant enhancement of N-methylnicotinamide (NMN) uptake by renal cortical slices when measured 24 h after a singleExpand
Inhibition of transmethylations of biogenic amines by S-adenosylhomocysteine. Enhancement of transmethylation by adenosylhomocysteinase.
The results show that S-adenosylhomocysteine, a product from S- adenosylmethionine, is a potent inhibitor of these methyltransferases, and that the stimulating factor in brain is an enzyme which enhances transmethylations by hydrolyzing S-ADenosylHomocysteines. Expand
Reversal of malignant transformation by tumor DNA.
Walker-256 carcinosarcoma cells grown in tissue culture medium containing inhibitory DNA prepared from the tumor are shown to have an unaltered growth in vitro, but a diminished capacity to produceExpand
A fluorimetric method for quantitation in the picomole range of N1-methylnicotinamide and nicotinamide in serum.
Abstract A method is described for the fluorimetric determination of N 1 -methylnicotinamide in deproteinized serum extract and of nicotinamide after extraction into ethyl acetate from deproteinizedExpand
  • H. Gaylord
  • Medicine
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  • 1913
Transfusion and Serum Reaction in Carcinoma and Sarcoma and Observations on the Hemolytic Reactions of the Blood in Dogs Affected with Transplantable Lymphosarcoma. Expand
Nicotinamide: a natural inhibitor of tRNA methylase.
Kinetic evidence indicates that nicotinamide is an inhibitor of tRNA methylase, and chromatographic and electrophoretic data as well as ultraviolet and mass spectral data indicate this. Expand
A procedure for gel filtration of viscous solutions
Abstract A procedure for gel filtration of viscous solutions with Sephadex® G-25 beads has been developed with the following characteristic features. A bed of such beads is packed and the liquid inExpand
The Inhibition of tRNA Methylase Activity by Nicotinamide and a 1732 CANCER
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J. Biol. Chem
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