Alterations on growth and cell organization of Giardia intestinalis trophozoites after treatment with KH-TFMDI, a novel class III histone deacetylase inhibitor.

  title={Alterations on growth and cell organization of Giardia intestinalis trophozoites after treatment with KH-TFMDI, a novel class III histone deacetylase inhibitor.},
  author={Ana Paula Rocha Gadelha and B{\'a}rbara de Gaspar Bravim and Juliana C Vidal and Lissa Catherine Reignault and Bruno Cosme and Kilian V. M. Huber and Franz Bracher and Wanderley de Souza},
  journal={International journal of medical microbiology : IJMM},
  volume={309 2},
8 Citations
Antimicrobial resistance of the enteric protozoon Giardia duodenalis – A narrative review
Salvage strategies including various options for combination therapy exist in spite of limited evidence and lacking routine diagnostic-compatible assays for antimicrobial susceptibility testing in G. duodenalis.
Nanoarchitecture of the ventral disc of Giardia intestinalis as revealed by high-resolution scanning electron microscopy and helium ion microscopy
Results showed that the disc is a non-uniformly organized structure that presents specific domains, such as the margin and the ventral groove region, and actin-like filaments and microtubules of the disc are associated, showing an interconnection between elements of the cytoskeleton of the trophozoite.
Discovery of Benzopyrrolizidines as Promising Antigiardiasic Agents
This compound produced morphologic modifications of trophozoites, with occasional loss of one of the nuclei, among other changes not observed with standard giardicidal drugs, suggesting that it might act through a novel mechanism of action.


KH-TFMDI, a novel sirtuin inhibitor, alters the cytoskeleton and mitochondrial metabolism promoting cell death in Leishmania amazonensis
Physiological studies for mitochondrial function, flow cytometry with propidium iodide and TUNEL assay confirmed the alterations in the mitochondrial metabolism, cell cycle, and DNA fragmentation, respectively, which could result to cell death by mechanisms related to apoptosis-like.
Inhibition of NAD+-dependent histone deacetylases (sirtuins) causes growth arrest and activates both apoptosis and autophagy in the pathogenic protozoan Trypanosoma cruzi
The effects of a novel 3-arylideneindolin-2-one that inhibits sirtuins are reported, which are highly conserved proteins that are involved in a variety of physiological processes and highlighted as a potential new target for Chagas disease therapy.
The effects of the antiprotozoal drugs metronidazole and furazolidone on trophozoites of Giardia lamblia (P1 strain)
Furazolidone was more effective than metronidazole and its effects were observed in cells treated with 1 µg/ml (the lowest concentration) as early as 6 h after the start of exposure, showing the cytoplasm depleted of its contents and great changes in volume.
Overexpression of Cytoplasmic TcSIR2RP1 and Mitochondrial TcSIR2RP3 Impacts on Trypanosoma cruzi Growth and Cell Invasion
The data suggest that sirtuin activity is important for the proliferation of T. cruzi replicative forms, for the host cell-parasite interplay, and for differentiation among life-cycle stages; but each one performs different roles in most of these processes.
Programmed cell death in Giardia
It is proposed that Giardia possess a pathway of autophagy and a form of apoptosis very different from the classical known mechanism; this may represent an early form of programmed cell death.
In vitro resistance to 5-nitroimidazoles and benzimidazoles in Giardia duodenalis: variability and variation in gene expression.