Alterations in number of protofilaments in microtubules assembled in vitro

  title={Alterations in number of protofilaments in microtubules assembled in vitro},
  author={G B Pierson and Paul R. Burton and Richard H. Himes},
  journal={The Journal of Cell Biology},
  pages={223 - 228}
Tubulin from bovine brain was polymerized in vitro using a variety of assembly conditions. Many of the formed microtubules were shown to contain 14 wall protofilaments. The number of microtubules containing 14 protofilaments increased with consecutive repetitions of cold-dissociation followed by reassembly in vitro. 

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