Alterations in linker flexibility suppress DNA topoisomerase I mutant-induced cell lethality.

@article{Losasso2007AlterationsIL,
  title={Alterations in linker flexibility suppress DNA topoisomerase I mutant-induced cell lethality.},
  author={Carmen Losasso and Erica Cretaio and Komaraiah Palle and Luca Pattarello and M. -A. Bjornsti and Piero Benedetti},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 13},
  pages={9855-64}
}
Eukaryotic DNA topoisomerase I (Top1p) catalyzes changes in DNA topology via the formation of a covalent enzyme-DNA intermediate, which is reversibly stabilized by the anticancer agent camptothecin (CPT). Crystallographic studies of the 70-kDa C terminus of human Top1p bound to duplex DNA describe a monomeric protein clamp circumscribing the DNA helix. The structures, which lack the N-terminal domain, comprise the conserved clamp, an extended linker domain, and the conserved C-terminal active… CONTINUE READING

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