Alteration of the product specificities of prenyltransferases by metal ions.

  title={Alteration of the product specificities of prenyltransferases by metal ions.},
  author={Shin-ichi Ohnuma and Takako Koyama and Kyozo Ogura},
  journal={Biochemical and biophysical research communications},
  volume={192 2},
Effect of several kinds of metal ions on the chain-length distribution of reaction products of polyprenyl diphosphate synthases was investigated. In the presence of Co2+ or Mn2+ octaprenyl-, solanesyl- and decaprenyl diphosphate synthases gave a variety of polyprenyl products with the longest chains being shifted by one or two isoprene units longer than those of the products formed in the presence of Mg2+. Thus octaprenyl diphosphate synthase became to give solanesyl (C45) and decaprenyl (C50… 
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Isoprenyl diphosphate synthases: the chain length determining step in terpene biosynthesis
A review of the recent developments in the study of isoprenyl diphosphate synthases with an emphasis on analytical techniques, product length determination, and the physiological consequences of manipulating expression in planta discusses analytical techniques for the determination of product length and the factors that control product length.
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Farnesyl diphosphate synthase; regulation of product specificity.
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Dimer Formation of Octaprenyl-diphosphate Synthase (IspB) Is Essential for Chain Length Determination of Ubiquinone*
Results indicate that although the A79Y mutant is functionally inactive, it can regulate activity upon forming a heterodimer with wild-type IspB, and this dimer formation is important for the determination of the isoprenoid chain length.
The regulatory function of isoprenyl diphosphate synthases in terpene biosynthesis
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An alternative cis-isoprenyltransferase activity in yeast that produces polyisoprenols with chain lengths similar to mammalian dolichols.
The results establish that yeast contains at least two cis-IPTases, and indicate that the chain length of dolichols is determined primarily by the enzyme catalyzing the chain elongation stage of the biosynthetic process.
Presence of copalyl diphosphate synthase gene in an actinomycete possessing the mevalonate pathway.
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